Journal article
Red blood cells polarize green laser light revealing hemoglobin's enhanced non-fundamental Raman modes
KM Marzec, D Perez-Guaita, M De Veij, D McNaughton, M Baranska, MWA Dixon, L Tilley, BR Wood
Chemphyschem | WILEY-V C H VERLAG GMBH | Published : 2014
Abstract
In general, the first overtone modes produce weak bands that appear at approximately twice the wavenumber value of the fundamental transitions in vibrational spectra. Here, we report the existence of a series of enhanced non-fundamental bands in resonance Raman (RR) spectra recorded for hemoglobin (Hb) inside the highly concentrated heme environment of the red blood cell (RBC) by exciting with a 514.5 nm laser line. Such bands are most intense when detecting parallel-polarized light. The enhancement is explained through excitonic theory invoking a type C scattering mechanism and bands have been assigned to overtone and combination bands based on symmetry arguments and polarization measuremen..
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Funding Acknowledgements
B.R.W. is supported by an Australian Research Council (ARC) Future Fellowship (FT120100926) and the instrument was purchased through an ARC Linkage Infrastructure Equipment and Facilities (LEIF) grant (LE100100215). This work is supported, in part, by an ARC grant DP140102504 and by the European Union from the resources of the European Regional Development Fund under the Innovative Economy Program (Grant coordinated by JCET-UJ, No. POIG.01.01.02-00-069/09). K.M.M. acknowledges financial support from a Go8 Fellowship, enabling a research stay at Monash University and the Polish National Science Center (NCN) (grant no. UMO-2012/07/D/ST4/02214). D.P.G. acknowledges financial support from the grant "Segles V" provided by the University of Valencia, enabling a research stay at Monash University. We thank Finlay Shanks (Monash University) for instrumental support.